This book is a practical companion in biochemistry to students of MBBS and other allied sciences, and for those engaged in biochemistry laboratories. Completely revised and updated, this new edition presents the subject in a lucid style and can be easily assimilated. The language is simple and easy to understand. The book contains relevant illustrations, tables and boxes, making it reader-friendly. The content is organised into 31 chapters of qualitative analyses and quantitative estimations, covering principles, procedures and observations followed by clinical interpretation. Specially formulated MCQs, problem-based exercises, questions relating to interpretation of laboratory data and case studies are provided to help students prepare for competitive examinations in India and abroad.
Rafi , MBBS MD FIMSA, is Professor of Biochemistry at Dr NTR University of Health Sciences, Andhra Pradesh.
Preface to the Second Edition
Preface to the First Edition
QUALITATIVE EXPERIMENTS
1. Safety in the Laboratory
2. Reactions of Carbohydrates
Reactions of Glucose
Reactions of Fructose
Reactions of Maltose
Reactions of Lactose
Reactions of Sucrose
Identification of Unknown Carbohydrate
3. Reactions of Lipids
Reactions of Triacylglycerols
Reactions of Cholesterol
4. Reactions of Proteins
Precipitation Reactions of Proteins
Colour Reactions of Proteins
Reactions of Albumin
Reactions of Casein
Reactions of Gelatin
Reactions of Peptone
Identification of Unknown Protein
5. Reactions of Non-protein Nitrogenous (NPN) Substances
Urea
Uric Acid
Creatinine
Identification of Unknown NPN Substance
6. Identification of Unknown Substance of Biochemical Importance
Carbohydrates, Proteins and NPN Substances
7. Analysis of Urine
Physical Examination
Microscopic Examination
Chemical Analysis
8. Analysis of Abnormal Constituents in Urine
Glucose
Ketone Bodies
Proteins
Blood
Bile Salts
Bile Pigments
Identification of Unknown Abnormal Constituents in Urine
9. Qualitative Analysis of Saliva
10. Qualitative Analysis of Gastric Juice
11. Qualitative Analysis of Bile
12. Qualitative Analysis of Milk
13. Food Analysis
QUANTITATIVE EXPERIMENTS
14. Introduction to Clinical Biochemistry
Glassware and Instruments Used in the Laboratory
Solutions
Sample Collection and Handling
Principles of Colorimetry
Quality Control
15. Blood Glucose
16. Glucose Tolerance Test
17. Blood Urea and BUN
18. Serum and Urinary Creatinine
19. Uric Acid
20. Serum Total Proteins and Albumin–Globulin Ratio
21. Cholesterol and Lipid Profile
22. Bilirubin
23. Aminotransferases (AST and ALT)
24. Alkaline and Acid Phosphatases (ALP and ACP)
25. Creatine Kinase and its Isoenzymes
26. Amylase and Lipase
27. Calcium and Phosphorus
28. Serum Electrolytes 171
29. Quantitative Analysis of CSF
30. Electrophoresis
31. Chromatography
32. Spectroscopic Examination of Hemoglobin and its Derivatives
33. Radioimmunoassay and Enzyme-linked Immunosorbant Assay