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Molecular Biotechnology: Principles and Practices is intended as a textbook aimed at providing undergraduate and postgraduate students with a strong base in this emerging and highly promising interdisciplinary science. It strikes a balance between two important aspects of the science – the theory of molecular biology and the experimental approach to the study of biological processes. The main feature of this book is that it covers a wide range of molecular techniques in biotechnology and is designed to be a student- and teacher-friendly textbook. Each technique is described conceptually, followed by a detailed experimental account of the steps involved. The book can also serve as reference to the interested reader who is venturing into the field of biotechnology for the first time. Special Features-Provides comprehensive and up-to-date coverage of key concepts in biotechnology- Logical format used to provide easy access to the information - Clear and well-labelled figures - Extensive cross–referencing between chapters
Channarayappa received his B.Sc. (Ag.), M.Sc. (Ag.), and Ph.D. degrees from the University of Agricultural Sciences, Bangalore. He then went on to earn a second Ph.D., in Genetics and Developmental Biology from West Virginia University, USA, in 1990. He worked as a Postdoctoral Fellow and Postdoctoral Research Associate at the University of Texas MD Anderson Cancer Center, Houston. He was the recipient of the Jawaharlal Nehru National Award for Outstanding Post-graduate Agricultural Research (1993) for significant research contribution in genetics and plant breeding, awarded by the Indian Council of Agricultural Research, New Delhi. He has been teaching both undergraduate and postgraduate students of molecular biology and biotechnology courses for more than 20 years. He is the author of many scientific papers resulting from research conducted in plant sciences, mutation research, and cancer biology.
PART I: Introduction to Biotechnology 1. Biotechnology: Scope and Importance 2. Biosafety and Good Laboratory Practices PART II: Advanced Techniques in Molecular Biology 3. Techniques of Cell Fractionation and Centrifugation 4. Chemical Synthesis of Nucleic Acids 5. DNA Chip Technology and its Potential Applications 6. Bioinformatics in Biotechnology PART III: Working with Nucleic Acids 7. Isolation of Nucleic Acids 8. Measuring Nucleic Acid Concentration and Purity 9. Electrophoretic Techniques 10. DNA Sequencing 11. Genetic Maps and Marker Analysis 12. Polymerase Chain Reaction (PCR) 13. In Situ Hybridization PART IV: Recombinant DNA and Genetic Engineering 14. Fundamentals of Recombinant DNA Technology 15. Enzymes in Molecular Cloning 16. Gene Constructs and Cloning Vectors 17. DNA Libraries 18. Molecular Biology of Gene Transfer Systems 19. Selection and Screening of Recombinant Molecules PART V: Applications of Biotechnology 20. Genetic Engineering of Microorganisms 21. Genetic Engineering of Animals 22. Genetic Engineering in Plants PART VI: Working with Proteins 23. Protein Purification Techniques 24. Protein Detection and Estimation 25. Protein Fractionation Techniques 26. Immunochemical Techniques PART VII: Bacterial and Mammalian Cell Culture 27. Biology of Bacteria 28. Cultivation of Mammalian Cells In vitro PART VIII: In Vitro Plant Cell Culture and Crop Improvement 29. Plant Cell Culture Laboratory and Requirements 30. Plant Culture Media, Preparation, and Culture Initiation 31. Micropropagation 32. Cultures of Organized Tissues 33. Culture of Unorganized Tissues 34. Cryopreservation and Distribution of Clonal Material 35. Measurement of Plant Cell Growth and PART VIII: Cytological Analysis 36. Protoplast Fusion and Somaclonal Variation 37. Application of Plant Cell, Tissue and Organ Culture PART IX: Environmental Biotechnology 38. Biotechnology in Pollution Control 39. Biodiversity and Genetic Conservation 40. Bioenergy Fuel from Biomass 41. Regulatory Aspects of Using Genetically-Modified Organisms 42. Intellectual Property Rights and Socio-Legal Aspects of Biotechnology Appendices husband cheated on me why husband cheat on their wife signs of infidelitymeet and cheat reason women cheat linkmarried men cheat with men when women cheat read herewhy married men have affairs my wife cheated open